Pramipexole Hydrochloride is a nonergot dopamine agonist, indicated for the treatment of the Parkinson''s disease. An attempt was done to develop simple precise, accurate and validated HPTLC method for the estimation of Pramipexole Hydrochloride bulk and in pharmaceutical dosage form. The compounds were separated on aluminium-backed silica gel 60 F254 plates with toluene, acetonitrile, methanol, ammonia, 5.5:3:1.5:0.2 (% v/v/v/v) as mobile phase. These system was found to give compact spots for the drug with the RF value of 0.50. Densitometric analysis of Pramipexole Hydrochloride was done at 268 nm. Regression analysis for the calibration plots was indicative of good linearity between response and concentration over the range 2000-6000 ng per spot with the correlation coefficient (r2) 0.9991. Pramipexole Hydrochloride was subjected to acid, base, peroxide, dry heat and photo degradation. It was susceptible to acid and base hydrolysis, oxidation, dry heat and photodegradation. Statistical analysis proved the method is repeatable, selective, and accurate for estimation of Pramipexole Hydrochloride. Because the method could effectively separate the drugs from their degradation products, it can be used as a stability-indicating method.
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